MB 543 Maleimide

MB™543 is a very hydrophilic, water-soluble orange fluorescent dye.Characteristic features of MB™543 are strong absorption,high fluorescence quantum yield,high photostability, and pH insensitivity from pH 3 to pH 10. MB™543 is a highly suitable dye for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM,STED etc.Maleimide is the most popular sulfhydryl-reactive reactive group for conjugating the dye to a thiol group on a protein, oligonucleotide thiophosphate,or low molecular weight ligand.The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups,–SH) at pH 6.5 to 7.5 to form stable thioether bond. The resulting conjugates exhibit brighter fluorescence and greater photostability than the conjugates of many other spectrally similar fluorophores. 

msds of MB 543 Maleimide